说明: 说明: 说明: 说明: C:\Users\doctorM\Desktop\circos.png

SESAME GENOME  The fine genome of S. indicum L., var. Yuzhi 11 (PRJNA74261) has been constructed using the complicated ‘Illumina + Roche 454 + BAC paired-end (ABI3730) + PacBio’ sequencing platform and the ‘SNP genetic map + BAC-FISH physical map + Optical map’ assisted assembly method (Table 1). The final genome assembly reaches 335.9Mb, occupying 94.8% of the 354Mb genome estimated by K-mer. In the fine genome map, the number of total scaffolds is 3,734, most of which are distributed into the 13 chromosomes of sesame karyotype. The number of N50 scaffolds is 18 and the N50 scaffold size is 6.38Mb. The number of N90 scaffolds is 886 and the N90 scaffold size is 19.6Kb. The largest scaffold covers 14.1Mb. With the aid of ultra-density SNP genetic linkage map carrying 30,194 unique SNPs and the physical map harboring 210 BACs, the fine genome map has been constructed. In the sesame genome, a total of 31,462 gene models were found in the sesame genome and 17% are related to fatty acid synthesis and metabolism.

 

Table 1  Genome assembly of S. indicum L. using complicated sequencing platform and assembly strategy

Genome assembly

Illumina Pair-end  and Meta-pair a

Illumina and Roche 454 b

PacBio-Only (100X)

Illumina+ Roche 454+ PacBio +  Opical mapping

All Scaffolds

Total base

231,504,252

261,976,889

325,173,333

335,922,006

Largest length

238,137

8,353,280

6,723,434

14,138,935

No.of scaffolds

22,749

4,633

5,728

3,729

N50 scaffold

2,885

45

81

18

N50 length

22,671

1,709,299

1,023,035

6,383,290

N90 scaffold

11,648

197

2,021

886

N90 length

4,307

218,088

14,427

19,645

GC content

34.632%

35.180%

35.180%

35.754%

 

N rate

0.139%

17.790%

0.000%

7.350%

All Contigs

Total base

229,184,095

214,949,860

325,173,333

311,229,756

Largest length

238,137

149,839

6,723,434

7,650,864

No. of contigs

24,594

20,040

5,728

3,912

N50 contig

3,405

3,082

81

60

N50 length

19,043

19,484

1,023,035

1,381,074

N90 contig

13,324

12,094

2,021

1,192

 

N90 length

3,960

4,361

14,427

18,236

a The libraries with the insert sizes of 500bp, 3kb and 5kb, respectively.

b The libraries with the insert sizes of 500bp, 3kb, 5kb, 10kb and 20kb, respectively.